Our team focus on studying the plant and nematode interaction using Multi-Omics analysis, and developing the enviromental-friendly nematode control strategies. Plant parasitic nematodes (PPNs) are the agronomically important pest that cause severe crop losses and economic damage worldwide. Due to the non-specific and slowly developing symptoms of PPN infection in the aboveground of host, the infection of PPN are often overlooked. In addition, the high dosage of toxic nematicides brings significant negative effects on environment, ecosystem and human health. Therefore, we aim to integrate the mass spectrometry (MS)-based Omics technologies, including proteomics, peptideomics and metabolomics analysis to reveal the signal transduction and regulatory mechanism of plant-nematode interaction. Our research can help us to find the molecular markers for early diagnosis of nematode invasion and further facilitate the development of new methods for nematode management.