Entry number:27
SUBJECT
Developing a Silver Nanoparticle-incorporated Antimicrobial Electrospinning Fiber based wound dressing by Argon-plasma
DEPARTMENT
Department of Medical Laboratory Science and Biotechnology
PROFESSOR AND STUDENT
Professor:In-Gann Chen, Changshu Kuo, and Wei-Lun Huang
Student:Wen-Hsiu Wang
ABSTRACT
By the method “plasma surface bombardment”, more silver nanoparticles (AgNPs) can be exposed to the surface of AgNPs-incorporated electrospinning, which increases the contact area with bacteria while still maintaining a good binding interaction between the AgNPs and the electrospinning polymer. To properly evaluate the antimicrobial ability of AgNPs-incorporated electrospinning in the liquid phase and define our wound dressing product, we refer to nanoMark (Taiwan) and conducted the experiments modified by nanoMark TN-013, JIS L1902/ISO 20743 and AATCC-100 standards, comparing the antimicrobial ability of AgNPs-incorporated electrospinning and commercial Ag containing wound dressing. In addition to testing the antimicrobial ability of the intact wound dressing, we also made both our product and the commercial one washed for 72 hours and evaluated the antimicrobial ability of them that could demonstrate the stability and durability of the products. The result shows that before washing, the antimicrobial efficacy of our AgNPs-incorporated electrospinning is 99.9%, while that of commercial one is 91.2%; after washing, our AgNPs-incorporated electrospinning still retain high antimicrobial efficacy of 97.1%, while the commercial wound dressing has a significant ten percentage reduction to 81.4%. Therefore, the method “plasma surface bombardment” not only increases the antimicrobial potency under the initial environment, but also solve the problem of one-time release of silver and achieve a stable sterilization effect. In addition, our research team also won the second place of trial of International Nanotechnology Olympiad with this project.